Bio-Rad QX600 Droplet Digital PCR System

                                                                         Requirements

The laboratory requires a digital droplet PCR (ddPCR) platform capable of absolute nucleic acid quantification with high sensitivity and multiplex capability. 

The quoted system provides up to six-color multiplex detection, processes up to 96 samples per run, and performs absolute nucleic acid quantification 

Type of Order:  

Firm Fixed Price Purchase Order 

Special Order Requirements:  

The instrument shall: 

• Perform digital droplet PCR using partitioned droplet technology.  

• Partition each 20 μL reaction into approximately 20,000 droplets.  

• Support six-color fluorescence detection.  

• Process 96-well plates.  

• Provide absolute nucleic acid quantification without reliance on standard curves.  

• Support applications including:  

• mutation detection  

• copy number variation  

• gene expression  

• viral titer analysis  

• Include software capable of automated thresholding, Poisson analysis, multiplex analysis, and export of results.  

• Include all required computer hardware and system software.  

• Include a compatible thermal cycler capable of operating 96-well deepwell PCR plates.  

These specifications are consistent with the manufacturer's published technical specifications. 

The contractor shall deliver the following equipment to the National Institutes of Health (NIH): 

• One (1) Bio-Rad QX600 Droplet Digital PCR System  

• One (1) Bio-Rad PTC Harmony Deepwell Thermal Cycler  

Training:  

Training is not included under this purchase. The contractor is not required to provide user training as part of this award.